42-MG-BA

Cat.No.: CSC-C0466

Species: Homo sapiens (Human)

Source: Brain

Morphology: flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes

Culture Properties: monolayer

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Cat.No.

CSC-C0466

Description

Established from the temporal lobe brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to treatment)

Species

Homo sapiens (Human)

Source

Brain

Recommended Medium

80% mixture of RPMI-1640 + EMEM (at 1:1) + 20% h.i. FBS

Culture Properties

monolayer

Morphology

flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes

Karyotype

Human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2, dup(2)(p12p2?4)a

Disease

Glioblastoma

Quality Control

Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA: revers

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.