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42-MG-BA
- Specification
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Cat.No.
CSC-C0466
Description
Established from the temporal lobe brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to treatment)
Species
Human
Source
glioma
Culture Properties
monolayer
Morphology
flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes
Karyotype
Human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2, dup(2)(p12p2?4)a
Quality Control
Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA: revers
Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
Viruses: ELISA: revers
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What is the storage and transport environment for the cells?
A:
Stored in liquid nitrogen and transported on dry ice.
Average Rating: 5.0 | 1 Scientist has reviewed this product
Helpful
I have learnt a lot from the Creative Bioarray website and it has been very helpful for my experiments.
29 Mar 2022
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