8-MG-BA

Cat.No.: CSC-C0467

Species: Homo sapiens (Human)

Source: Brain

Morphology: epithelial-like, adherent cells growing as monolayer

Culture Properties: monolayer

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Cat.No.

CSC-C0467

Description

Established from the frontal brain lobe of a 54-year-old woman with glioblastoma multiforme in 1989 (pre-treatment)

Species

Homo sapiens (Human)

Source

Brain

Recommended Medium

80-85% EMEM + 15-20% h.i. FBS

Culture Properties

monolayer

Morphology

epithelial-like, adherent cells growing as monolayer

Karyotype

Human flat-moded hyperdiploid karyotype with 15% polyploidy - 52(47-52)<2n>X, -X, +1, +1, +3, +5, +6, +7, +8, -19, +2-4mar, add(1)(p34-36;hsr), add(1)(p34-36), der(1)t(1;17)(q11-12;q12-21), der(3;5)(q10;q10), i(5p), del(6)(q24-25), del(7)(p14), i(7p), i(8

Disease

Glioblastoma

Quality Control

Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin (+), cytokeratin-7 -, cytokeratin-8 (+), cytokeratin-17 -, cytokeratin-18 -, cyto

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.