Porcine Pulmonary Artery Fibroblasts

Specification
Recommended Products

Cat.No.

CSC-C4899L

Description

Porcine Pulmonary Artery Fibroblasts from Creative Bioarray are isolated from pulmonary artery of porcine. Porcine Pulmonary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution and incubated in Creative Bioarray Complete Growth Medium. The cells are shipped at passage 2. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen.

Species

Porcine

Source

Lung; Artery

Recommended Medium

SuperCult® Porcine Pulmonary Artery Fibroblast Medium

Cell Type

Fibroblast

Disease

Normal

Quality Control

Porcine Primary Pulmonary Artery Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Porcine Primary Pulmonary Artery Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

Is digestion critical?

The poor state of the cells is many times closely related to tryptic digestion during passaging. Generally tumor cells can be digested for 1-2min. However, each kind of cell has a different ability to adhere to the wall, so the response to trypsin is also different, we need to follow closely. Generally, it can be terminated when the naked eye observes that the adherent cell layer can move; rather than the cells are all dispersed into a single one.

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Average Rating: 4.0 | 1 Scientist has reviewed this product

Continued support

Working with Creative Bioarray has been an absolute pleasure, I will continue to use them in the future.

12 Aug 2023

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