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TK-6

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Cat.No.
CSC-C0698
Description
established 1995 from the pleural effusion of a 30-year-old man with chronic myelogenous leukemia (CML) in T cell lineage blast crisis after bone marrow transplantation; cells carry the Philadelphia chromosome with a e14-a2 (b3-a2) BCR-ABL1 fusion gene. TK6 cells are CD19 positive, 50% of the cell population express CD20 and a small sub-population has been tested positive for CD22. This cell line has been shown to be negative for Bovine Viral Diarrhoea Virus (BVDV).
Organism
Homo sapiens, human
STR DNA Profile
Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20
Karyotype
human hyperdiploid karyotype; 48(46-50)<2n>XY, +22, +22, del(1)(q24), del(6)(q21q24), dup(7)(p1?4q3?5)inv(7)(p14q35), t(9;22)(q34;q11), add(21)(p11), der(22)?inv(22)(p11q11)t(9;22)(q34;q11)x2; carries complex t(9;22) effecting BCR-ABL1 rearrangement with 3x genomic amplification in der(22); matches published karyotype
Source
lymphoblast
Culture Properties
Suspension
Morphology
round cells growing singly in suspension
Recommended Medium
Quality Control
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
liquid nitrogen vapor phase
Disease
chronic myeloid leukemia
Application
This cell line can be used in bromodeoxyuridine resistant assays.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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