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GF-D8
- Specification
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Cat.No.
CSC-C0614
Description
Established from the peripheral blood of an 82-year-old man with acute myelocytic leukemia (AML M1) at diagnosis in 1989; described to be growth-dependent on granulocyte-macrophage colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3)
Species
Human
Source
acute myelocytic leukemia
Recommended Medium
Culture Properties
suspension
Morphology
round to polymorph cells growing in suspension
Karyotype
Human hyperdiploid karyotype; 49(45-51)<2n>XY, +8, +13, +21, +mar, der(5)t(5;15)(q11;q25), der(7)t(7;15)(q31;q24), ?inv(7)(q31.3q35)x2, der(8)ins(8;11)(q24;q23q24)dup(8)(q23q24)dup(11)(q22q23), der(11)t(8;11)(q24;q23)dup(8)(q23q24)dup(11)(q22q23), der(11;
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, HLA-DR -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Immunology: CD3 -, CD13 +, CD14 -, CD15 -, CD19 -, CD33 +, CD34 -, HLA-DR -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Q:
What is the importance of karyotyping in cell culture?
A:
Karyotyping, traditionally performed using cytogenetic techniques, is indispensable for validating genome assemblies whose sequence lengths can be scaled up to chromosome sizes using modern methods.
Average Rating: 5.0 | 1 Scientist has reviewed this product
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The ease of ordering and timely delivery of Creative Bioarray has expedited my experimental process.
15 Feb 2023
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