Human Hypertension Artery Endothelial Cells

Specification
Recommended Products

Cat.No.

CSC-C4984J

Description

Human hypertension artery endothelial cells from Creative Bioarray are isolated from the carotid artery of human donor that has been diagnosed with hypertension. Human hypertension artery endothelial cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 2 min and incubated complete growth medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, Cells at passage 3 are detached from flasks and immediately cryopreserved in vails. Each vial contains at least 0.5×10^6 cells per ml.

Species

Human

Source

Artery

Recommended Medium

SuperCult® Human Endothelial Cell Growth Medium Kit

Cell Type

Endothelial

Disease

Diseased

Quality Control

Human hypertension artery endothelial cells are negative for bacteria, yeast, fungi and mycoplasma.

Storage and Shipping

Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1464J

SuperCult® Human Endothelial Cell Growth Medium Kit

INQUIRY

For research use only. Not for any other purpose.

Q & A
Customer Review

The black spot has been created, how do I proceed?

If it is suspension cells, collect the cell supernatant and centrifuge it slowly (500-600 rpm/min, 5-6 min) and replace it with a new culture flask. If it is wall-adherent cells, wash the cells with PBS for 2-3 times, when washing, gently tap the flask to dislodge the fragments and particles that are not firmly adhered to the wall, and then discard the PBS, digest the cells by adding low concentration trypsin such as 0.05% trypsin for 1 min or so, let the particles and fragments in the cell gap fall off, remove the low concentration trypsin, and then digest the cells normally. around, let the particles and fragments in the cell gap fall off, remove the low concentration of trypsin, then digest the cells normally, centrifuge the collected cell suspension slowly (500-600 rpm/min, 5-6 min) and replace it with a new culture flask, and you can try to increase the concentration of serum appropriately for culture.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Good experimental results

We have had good experimental results with U87 / WT for brain cell research.

15 Jan 2021

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