Human Hypertension Coronary Artery Fibroblasts

Cat.No.: CSC-C4998J

Species: Human

Source: Artery

Cell Type: Fibroblast

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Cat.No.
CSC-C4998J
Description
Human hypertension coronary artery fibroblasts are isolated from the coronary artery of human donor that has been diagnosed with hypertension. Human hypertension coronary artery fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in complete growth medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 1 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml.
Species
Human
Source
Artery
Cell Type
Fibroblast
Disease
Diseased
Quality Control
Human hypertension coronary artery fibroblasts are negative for bacteria, yeast, fungi and mycoplasma.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use.
Never can cryopreserved cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
What happens when purchased cells die or have poor cell survival?

The common reasons for researchers to experience poor cell survival in cell culture can be categorized as follows. Incorrect use of medium or poor quality of medium. Serum is used incorrectly or the quality of the serum is poor. The transportation process has a serious effect on the cells. Wrong thawing process. Frozen cells are thawed, washed and centrifuged. Suspended cells are mistaken for dead cells. Incorrect use of culture temperature. Cells were left at -80℃ for too long.

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Average Rating: 5.0    |    1 Scientist has reviewed this product

Keen help

Many thanks to the technicians, who were eager to help us during the use of our NB16 cell products.

06 Dec 2022


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