Established from the normal liver of a 17-day rat embryo (strain JAR-2) in 1975
epithelial-like growth in monolayers
Rat hypertriploid karyotype with 13% polyploidy - 64(58-66)<3n> - large submetacentric marker present in most cells
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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