This cell line was derived from a 7-month-old female Wistar-Furth rat. GH3 cells produce growth hormone faster than the GH1 cell line and also produce prolactin. Hydrocortisone stimulates growth hormone production and inhibits prolactin production. Studies have shown that cells are capable of adaptation to suspension culture using MEM.
Rattus norvegicus, rat
Hyperdiploid, modal no. 60
Ham's F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% FBS
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
This cell line can be used as a suitable transfection host.
Rat Wistar-Furth pituitary tumour
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