Established from the liver tumor of a Buffalo rat; tumor cells were propagated in the hind leg
adherent, polymorph round cells growing in monolayers
Near-tetraploid rat karyotype with 17% polyploiy - 73-82<4n>
Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative
Storage and Shipping
Frozen with 70% medium, 20% horse serum, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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