Established from diethylstilbesterol-induced renal tumors of a 14-month-old male Syrian Golden Hamster in 1997; cells were described as expressing estrogen receptors, glial fibrillary acidic protein and other markers of the neuroectodermal lineage and as being responsive to estrogen
fibroblastoid cells growing in monolayers
hyperdiploid hamster karyotype with 10% polyploidy - s = 45(39-46)
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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