Established from diethylstilbesterol-induced renal tumors of a 14-month-old male Syrian Golden Hamster in 1997; cells were described as expressing estrogen receptors, glial fibrillary acidic protein and other markers of the neuroectodermal lineage and as being responsive to estrogen
hyperdiploid hamster karyotype with 10% polyploidy - s = 45(39-46)
fibroblastoid cells growing in monolayers
Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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