V-79

Cat.No.: CSC-C2756

Species: Cricetulus griseus, Chinese hamster

Source: Lung fibroblasts

Morphology: fibroblast

Culture Properties: adherent

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Cat.No.

CSC-C2756

Description

Established after spontaneous transformation of cells isolated from the lung of a normal Chinese hamster (male). The G1 phase in these cells is either absent or very short. Consequently they have been used in studies of the G1 growth period. V79 is well established in toxicology studies. Stability of karyotype and morphology is making them suitable for gene toxicity assays with low background aberrations. The cells do not express endogenous cytochrome P450 and have been used for the insertion and expression of P450 from human, rat, mouse and fish. The cells have a high plating efficiency (80%), and a generation time of 12 to 14 hours. The line was renamed V79 by Elkind in 1958.

Species

Cricetulus griseus, Chinese hamster

Source

Lung fibroblasts

Recommended Medium

DMEM + 10% h.i.FBS

Culture Properties

adherent

Morphology

fibroblast

Karyotype

2n=22

Application

This cell line can be used in studies of X-ray induced damage and repair processes.

Quality Control

Tests for mycoplasma, bacteria and fungi were negative

Storage and Shipping

liquid nitrogen vapor phase

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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