Established from the lung of a Syrian golden hamster fetus on day 15 after gestation in 1981; primary culture cells were treated with hydroxy-proline; cells were described as premature epithelial cells with the ability to differentiate into mucus-producing pulmonary cells
epithelial-like cells growing in monolayers
Near-diploid Syrian hamster karyotype with 10% polyploidy - 43-47<2n>
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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