This clone is a bromodeoxyuridine-resistant mutant derived from cell line C6 (established from a nitrosourea-induced brain tumor in a Wistar-Furth strain rat in 1971)
spindle-shaped, relatively small cells, growing adherently in monolayers
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 3-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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