Established from spleen and lymph nodes of an adult Maloney murine leukemia virus (MoMuLV)-infected female WMP/WMP wild mouse; cells are described to carry nine pairs of metacentric Robertsonian translocation chromosomes containing 1 to 18; cells can be used for physical gene mapping by in situ hybridization in mice
Murine karyotype with a preponderance of centric fusion markers - resembles published karyotype
single round cells growing in suspension
Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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