STC-1

STC-1 is a murine intestinal enteroendocrine cell line originally established in 1990 from an endocrine tumor of the small intestine in double-transgenic mice harboring rat insulin promoter-driven polyoma small T antigen and SV40 large T antigen transgenes. As one of the three most extensively characterized enteroendocrine cell models, STC-1 offers a uniquely plurihormonal secretory repertoire, producing and releasing cholecystokinin (CCK), secretin, glucose-dependent insulinotropic polypeptide (GIP), peptide YY (PYY), neurotensin, and proglucagon-derived peptides including GLP-1, GLP-2, and oxyntomodulin in a regulated, stimulus-dependent manner.

This broad hormonal profile closely mirrors the overlapping hormone expression patterns of native upper gastrointestinal enteroendocrine cells, making STC-1 exceptionally relevant for investigating gut-brain signaling, nutrient sensing, and metabolic regulation. The cells mount robust, dose-dependent secretory responses to diverse physiological stimuli—including monosaccharides, fatty acids, amino acids, and bioactive peptides—providing a reliable platform for high-throughput screening of food-derived bioactives, satiety modulators, and incretin secretagogues. With decades of peer-reviewed validation and standardized culture protocols, STC-1 remains the gold-standard murine model for mechanistic studies of intestinal hormone secretion and therapeutic discovery in metabolic disease.