FL83B

FL83B is an established murine hepatocyte cell line derived from normal fetal mouse liver at 15–17 days of gestation. The cells exhibit epithelial morphology and adherent growth, providing an expandable model for investigating selected aspects of hepatic biology. Although originating from normal tissue, FL83B cells are aneuploid and should be characterized as an established cell line rather than normal primary hepatocytes.

A distinctive advantage is the retention of several liver-associated metabolic and biosynthetic functions. FL83B cells have been reported to store glycogen, actively synthesize cholesterol, and produce serum proteins. Original characterization demonstrated synthesis of multiple mouse serum proteins, including albumin, under chemically defined culture conditions. These properties provide measurable endpoints for studying hepatic carbohydrate metabolism, lipid handling, and protein production within a controllable experimental system.

Another strength is its suitability for experimentally induced lipid-accumulation models. Oleic acid treatment produces intracellular lipid deposition that can be quantified using lipid staining and combined with viability, gene-expression, and protein-signaling measurements. FL83B studies have examined pathways involving AMPK, SREBP-1c, fatty acid synthesis, and lipid oxidation, supporting evaluation of candidate metabolic modulators. Its expandable culture format facilitates repeated experiments and comparisons across treatment conditions. With validation of the relevant endpoints, FL83B offers a practical platform for hepatic metabolism and cellular stress research; its fetal origin and established-line characteristics remain important when interpreting relevance to adult liver physiology.