NH15-CA2 is a hybrid cell line formed by the fusion of N115-BU-8 (a bromodeoxyuridine-resistant mutant of N1E-115 and 108CC15 (itself a mouse neuroblastoma N18TG2 x rat glioma C6-BU-1 hybrid line). This hybrid-hybrid line contains both cholinergic and adrenergic enzyme activities.
DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) HAT (0.1 mM hypoxanthine, 10 µM aminopterin and 16 µM thymidine) Cells can be cultured without HAT provided they were cultured inbetween in HT medium for 3-4 days
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cryopreserved cells be kept at -20 °C.
Liquid Nitrogen (-180 °C).
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