Established from the primary tumor (pT4N1M1) of a 56-year-old Caucasian man with esophageal adenocarcinoma in 2000; cells are described to carry a p53 mutation (C742T)
Human hypodiploid karyotype; 39(36-40)<2n>X, -Y, +2, -3, -5, -11, -12, -15, -16, -19, -20, -22, +mar add(X)(p22), del(1)(p32), add(12)(p13), der(13;21)(q10;q10), i(13)(q10), add(17)(p12), add(?18)(q21), add(21)(p11)
ovoid cells growing semiadherently, singly or in clumps
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 +, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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