Strain Balb/c Mouse Mesenchymal Stem Cells

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Scientific Data
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Cat.No.

CSC-C1300

Description

Balb/c mouse bone marrow mesenchymal stem cells are extracted from healthy Balb/c mouse bone marrow and have strong proliferation and multi-directional differentiation capabilities. It can be used as a cell model to study proliferation, aging, immunity, differentiation and transplantation.

Species

Mouse

Recommended Medium

Mouse Bone Marrow Mesenchymal Stem Cells Complete Medium

Application

For Research Use Only

Shipping

Dry Ice

Quality Control

Sterility, Safety, HIV/viruses, bacteria, fungi: negative.

Storage and Shipping

Remove cryovials (dry ice packaging) and place the vial into liquid nitrogen for storage. Alternatively, thaw and use the cells immediately.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Balb/c Mouse Mesenchymal Stem Cells (MSCs) are primary, multipotent stromal cells isolated from the bone marrow of the inbred Balb/c mouse strain. They exhibit the classical fibroblast-like, spindle-shaped morphology and adhere firmly to plastic surfaces. These cells are defined by their specific immunophenotype, uniformly expressing mesenchymal markers such as CD29, CD44, CD73, and Sca-1, while lacking hematopoietic lineage markers like CD11b, CD34, and CD45. Functionally, they possess robust trilineage differentiation potential, capable of differentiating into osteoblasts, adipocytes, and chondrocytes under specific inductive conditions.

Culturally, they are maintained in a nutrient-rich basal medium supplemented with serum and growth factors under standard conditions (37°C, 5% CO₂). Due to the genetic homogeneity of the Balb/c background, these MSCs offer high experimental reproducibility. They are widely utilized in tissue engineering, immunomodulation studies, and as a preferred model for in vivo homing assays, particularly in Balb/c-based xenograft or syngeneic transplantation systems.

Adipose-Derived Mesenchymal Stem Cells Attenuate Cutaneous Leishmaniasis Progression in BALB/c Mice

Leishmaniasis remains a major public health challenge, necessitating immunotherapies that activate macrophages and promote TH1 responses. While mesenchymal stem cells (MSCs) exhibit immunomodulatory effects, this study evaluated the impact of adipose-derived MSCs (AD-MSCs) on cutaneous leishmaniasis in BALB/c mice.

AD-MSCs displayed a fibroblast-like morphology and successfully differentiated into osteocytes and adipocytes (Fig. 1A-C). Immunophenotyping confirmed high expression of MSC markers (CD90: 44.68%, CD105: 61.55%, CD73: 46.82%, CD29: 95.7%) and low expression of hematopoietic markers (CD45: 1.54%, CD34: 1.12%) (Fig. 1D).

Following L. majorinfection, disease progression was monitored for 7 weeks. Infected control mice (Group II) developed lesions by week 2, with footpad thickness increasing significantly from weeks 3 to 7, culminating in necrosis. In contrast, AD-MSC-treated mice (Group I) exhibited delayed lesion formation with reduced severity. Footpad thickness in the treated group was significantly lower than in controls from weeks 4 to 7 (Fig. 2A). These findings demonstrate that AD-MSC administration attenuates disease progression in experimental cutaneous leishmaniasis.

Characterization of adipose-derived mesenchymal stem cells (AD-MSCs). — Fig. 1. Characterization of adipose-derived mesenchymal stem cells (AD-MSCs) (Zanganeh E, Soudi S, *et al*., 2020).

Evaluation of parasite proliferation and dissemination. — Fig. 2. Evaluation of parasite proliferation and dissemination (Zanganeh E, Soudi S, *et al*., 2020).

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For research use only. Not for any other purpose.