Mesenchymal Stem Cell medium-serum free (MSCM-sf) is designed for optimal growth and expansion of normal mesenchymal stem cells (MSCs) in vitro. MSCs can be expanded for several passages while maintaining their multipotential phenotype. It is a sterile, liquid medium which contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals. The medium is HEPES and bicarbonate buffered and has a pH of 7.4 when equilibrated in an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment at serum-free condition that selectively promotes growth of normal human mesenchymal stem cells in vitro.
Storage and Shipping
Store the basal medium at 4 °C, the MSCGS-sf and the P/S solution at -20 °C. Protect from light.
MSCM-sf consists of 500 ml of basal medium, 5 ml of mesenchymal stem cell growth supplement serum free and 5 ml of penicillin/streptomycin solution.
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