Introduction:HEKPlus SFM is a xeno-free, serum-free, defined medium optimized for suspension culture and transfection of human embryonic kidney (HEK 293) cells. HEK293Plus SFM promotes long-term, high density cell growth and supports recombinant protein or adenovirus production in HEK293 cells.
HEKPlus SFM should be supplemented with L-alanyl-glutamine or L-glutamine prior to use. The medium contains 0.1% Poloxamer 188, a surfactant shown to enhance cell yield and reduce cell aggregation. HEKPlus SFM also includes human transferrin and recombinant human insulin that have been shown to improve cell proliferation.
This medium has been optimized for use with HEK293T/17 SF suspension cells and GeneX Plus transfection reagent to reproducibly express a wide range of proteins at high levels. HEK293T/17 SF cells are pre-adapted to the medium and can be cultured in suspension and directly transfected in HEKPlus SFM using GeneXPlus transfection reagent. Transfection complexes can be prepared in the HEKPlus medium as well. In general, HEK293 cells currently adapted to serum-free medium can be subcultured directly using higher seeding densities. For cells currently adapted to serum containing medium, sequential adaptation to HEKPlus SFM is recommended.
Sterility tests are performed on each lot of medium using current USP methods. Each lot of medium is tested for the ability to support the growth of HEK 293 cell lines. Osmolality, pH, and endotoxin tests are also performed.
pH: 7.0 to 7.6
Storage and Shipping
Store at 2 °C to 8 °C. Do not freeze. Protect from light.
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