Apolipoprotein-D is mainly associated with high density lipoproteins in human plasma. Apolipoprotein-D is an atypical apolipoprotein and, based on its primary structure, is a member of the lipocalin family. Lipocalins adopt a beta-barrel tertiary structure and transport small hydrophobic ligands. Apolipoprotein-D binds cholesterol, progesterone, pregnenolone, bilirubin and arachidonic acid. Apolipoprotein-D is expressed in numerous tissues having high levels of expression in spleen, testes and brain. Apolipoprotein-D is present at high concentrations in the cyst fluid of women with gross cystic disease of the breast, a condition associated with increased risk of breast cancer. Apolipoprotein-D accumulates in regenerating peripheral nerves and in the cerebrospinal fluid of patients with neurodegenerative conditions, such as Alzheimers disease. Apolipoprotein-D participates in maintenance and repair within the central and peripheral nervous systems. Apolipoprotein-D is a multi-ligand, multi-functional transporter and transports a ligand from one cell to another within an organ, scavenge a ligand within an organ for transport to the blood or could transport a ligand from the circulation to specific cells within a tissue. Human Recombinant Apoliprotein-D produced in E. coli is a single, non-glycosylated, polypeptide chain containing 174 amino acids and having a molecular mass of 19.8 kDa. It has a His-tag fusion at the C-terminus (7 a.a.). The protein a.a.sequence corresponds to the UniProtKB/Swiss-Prot entry P05090. The following gene modifications were made: Trp99His, Cys116Ser, Ile118Ser, Leu120Ser amino acid exchanges were introduced at the surface of Apoliprotein-D to enhance the protein's solubility and another three Leu23Pro, Pro133Val, Asn134Ala amino acid exchanges which facilitate its genetic manipulation.
Human APOD expressed in E.coli
CAT# CSC-CTK0608-10 (10 μg); CAT# CSC-CTK0608-50 (50 μg)
Greater than 95% as determined by SDS-PAGE analysis.
Sterile-filtered, white, lyophilized (freeze-dried) powder. The protein was lyophilized from a concentrated (1 mg/ml) solution with in 4 mM KH ? PO ? , 16 mM Na ? HPO ? and 115 mM NaCl (pH 7.5).
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultrapure water or aqueous buffers to a concentration of 0.1- 0.5 mg mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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