COLO-849

Cat.No.: CSC-C0331

Species: Homo sapiens (Human)

Source: Lymph Node Metastasis

Morphology: fibroblastic cells growing mainly as adherent monolayer and partly in suspension; sedimented cells have a brown color (suggesting production of melanin)

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Cat.No.

CSC-C0331

Description

Derived in 1992 from right axillary lymph nodes of a 43-year-old Caucasian man with metastatic maligant melanoma

Species

Homo sapiens (Human)

Source

Lymph Node Metastasis

Recommended Medium

80-90% RPMI-1640 + 10-20% h.i. FBS

Morphology

fibroblastic cells growing mainly as adherent monolayer and partly in suspension; sedimented cells have a brown color (suggesting production of melanin)

Karyotype

Human hypotriploid karyotype - 60<3n>X, -Y, -1, +3, -4, -5, +7, -10, -11, -12, -13, -14, -15, -16, -17, -18, +20, +22, der(1)t(1;?)(q11;?), i(3q), idic(3)(3cen->p24::1q11->qter), der(7)t(7;?)(q33;?)x2, der(9)t(9;?)(p11;?)/del(9)(p13) - the large idic(3) m

Disease

Cutaneous Melanoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament -, vimentin +
Viruses: E

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

COLO 849; Colo 849; COLO #849; COLO849; Colorado 849

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

COLO-849 is a metastatic melanoma cell line from human origin first established in 1992. It originated from the right axillary lymph nodes of a 43 years old Caucasian male with metastatic malignant melanoma. It displays fibroblastic morphology and typically grows as an adherent monolayer with some suspension cells. Sedimented cells have a brown color which is attributed to melanin production, hence suggesting melanocytic origin.

Cells are maintained and cultured in RPMI 1640 supplemented with 10-20% fetal bovine serum at 37 °C and 5% CO₂ incubator. The doubling time for COLO-849 cells is approximately 50-60 hours. They are passaged every 4-7 days at a ratio of 1:4 to 1:10 with Trypsin/EDTA. Testing has shown this cell line to be reverse transcriptase negative. Other markers shown to be absent include EBV, HBV, HIV.

COLO-849 cell line has been used broadly in melanoma research. It can reliably be used as an in vitro model for metastatic melanoma biology, drug screening and therapeutic target validation.

LRIG1 Expression and Functional Effects in Melanoma Subtypes

Triple wild-type melanoma lacks MAPK/ERK pathway mutations and effective treatments, while LRIG1 regulates EGFR upstream of MAPK/ERK and PI3K/AKT pathways. Hadi et al. investigated whether recombinant LRIG1 inhibits proliferation in BRAF-mutated (A375) and triple wild-type (colo-489, wm-3211) melanoma cells.

LRIG1 mRNA expression was significantly higher in metastatic triple wild-type melanoma compared to BRAF-mutated, RAS-mutated, and NF1-mutated subtypes. Recombinant LRIG1 dose-dependently inhibited proliferation in colo-849 cells but not in A375 or wm-3211 cells, with wm-3211 even showing stimulation (Fig. 1). Western blot analysis revealed low endogenous LRIG1 expression across all lines, undetectable phospho-EGFR, and failed/inconclusive phospho-ERK detection; notably, LRIG1 treatment suppressed phospho-AKT in A375 cells but not in colo-849 or wm-3211 cells (Fig. 2A-F).

Comparison of cell proliferation among A375, colo-489, and wm-3211 cells cultured with or without indicated concentrations of control peptide His-MBP-Strep (control) or recombinant LRIG1 (sLRIG1). — Fig. 1. Comparison of cell proliferation among A375, colo-489, and wm-3211 cells cultured with or without indicated concentrations of control peptide His-MBP-Strep (control) or recombinant LRIG1 (sLRIG1) (Hadi M, 2021).

Protein extracts from A375, colo-489, and wm-3211 cell lines, except for Fig. D and F, which show protein extracts from colo-489 cells only. The green signal indicates LRIG1, P-EGFR, P-ERK, or P-AKT, while the red signal indicates actin detected with mouse anti-actin (1:3000) across all cell lines. — Fig. 2. Protein extracts from A375, colo-489, and wm-3211 cell lines, except for Fig. D and F, which show protein extracts from colo-489 cells only. The green signal indicates LRIG1, P-EGFR, P-ERK, or P-AKT, while the red signal indicates actin detected with mouse anti-actin (1:3000) across all cell lines (Hadi M, 2021).

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For research use only. Not for any other purpose.