Derived in 1992 from right axillary lymph nodes of a 43-year-old Caucasian man with metastatic maligant melanoma
Human hypotriploid karyotype - 60<3n>X, -Y, -1, +3, -4, -5, +7, -10, -11, -12, -13, -14, -15, -16, -17, -18, +20, +22, der(1)t(1;?)(q11;?), i(3q), idic(3)(3cen->p24::1q11->qter), der(7)t(7;?)(q33;?)x2, der(9)t(9;?)(p11;?)/del(9)(p13) - the large idic(3) m
fibroblastic cells growing mainly as adherent monolayer and partly in suspension; sedimented cells have a brown color (suggesting production of melanin)
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, GFAP -, HMB-45 +, neurofilament -, vimentin +
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 2-2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.