YNH-1

Specification
Recommended Products

Cat.No.

CSC-C0673

Description

Established from the peripheral blood of a 46-year-old man with acute myeloid leukemia (AML M1) at diagnosis in 1994; cells were described to be constitutively cytokine-dependent and cytokine-responsive and to carry the translocation t(16;21)(p11;q22) leading to FUS-ERG (TLS-ERG) fusion gene

Species

Human

Source

peripheral blood

Recommended Medium

60% RPMI-1640 + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 or 10 ng/ml G-CSF

Culture Properties

suspension

Morphology

single round cells growing in suspension, some cells are loosely adherent

Karyotype

Human hyperdiploid karyotype with 4% polyploidy; -47(44-48)<2n>XY, +19, add(7)(q35), der(16)t(1;16)(q12;q12)t(16;21)(p11;q22), der(21)t(16;21)(p11;q22; carries complex t(16;21) effecting rearrangement of FUS (TLS) with ERG; matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD20 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

CM-1002X

SuperCult® RPMI-1640 with L-glutamine & HEPES

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.