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YNH-1

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Cat.No.
CSC-C0673
Description
Established from the peripheral blood of a 46-year-old man with acute myeloid leukemia (AML M1) at diagnosis in 1994; cells were described to be constitutively cytokine-dependent and cytokine-responsive and to carry the translocation t(16;21)(p11;q22) leading to FUS-ERG (TLS-ERG) fusion gene
Organism
Human
Karyotype
Human hyperdiploid karyotype with 4% polyploidy; -47(44-48)<2n>XY, +19, add(7)(q35), der(16)t(1;16)(q12;q12)t(16;21)(p11;q22), der(21)t(16;21)(p11;q22; carries complex t(16;21) effecting rearrangement of FUS (TLS) with ERG; matches published karyotype
Source
peripheral blood
Culture Properties
suspension
Morphology
single round cells growing in suspension, some cells are loosely adherent
Recommended Medium
60% RPMI-1640 + 20% h.i. FBS + 20% vol conditioned medium of cell line 5637 or 10 ng/ml G-CSF
Quality Control
Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD4 +, CD13 +, CD14 -, CD15 -, CD19 -, CD20 -, CD33 +, CD34 +, cyCD68 +, HLA-DR -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/-II -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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CSC-6296W
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