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Cat.No.

CSC-C0591

Description

Derived from the bone marrow of a 50-year-old woman with B-acute lymphoblastic leukemia (B-ALL) in 1985; cells were described to carry the three-way-translocation t(8;14;18) and to overexpress the BCL2 and MYC genes

Species

Homo sapiens (Human)

Source

Bone Marrow

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

round to polymorph small cells, growing singly or in clumps in suspension

Karyotype

Human hyperdiploid karyotype - 48(44-50)<2n>XX, +8, +12, t(3;4)(q27;p11), der(8)t(8;14)(q24;q32)t(14;18)(q32;q21), ider(8)(q10)t(8;14)(q24;q32)t(14;18)(q32;q21), der(12)t(?1;12)(q25;q24), der(14)t(8;14)(q24;q32), der(18)t(14;18)(q32;q21) - sideline with d

Disease

Diffuse Large B-Cell Lymphoma

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 +, CD37 +, CD38 +, cyCD79a +, CD80 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -
Viruses: PCR: EBV +, HBV -, HCV -, HIV

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.