TOM-1

Specification
Q & A
Customer Review

Cat.No.

CSC-C0583

Description

Established in 1983 from the bone marrow of a 54-year-old woman with refractory Philadelphia chromosome-positive acute lymphoblastic leukemia (ALL); described to carry the ALL-variant (m-bcr) of the BCR-ABL1 fusion gene (e1-a2)

Species

Homo sapiens (Human)

Source

Bone Marrow

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

small round cells growing in suspension singly or in clumps, some cells are loosely adherent

Karyotype

Human hyperdiploid karyotype with 7% polyploidy - 47(47-48)<2n>X, -X, +8, +16, del(7)(p14), der(9)del(9)(q13q34)t(9;22)(q34;q11), der(22)t(9;22)(q34;q11) - carries t(9;22) effecting BCR-ABL1 rearrangement - resembles published karyotype

Disease

Adult B Acute Lymphoblastic Leukemia

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD2 -, CD3 -, CD4 -, CD7 -, CD10 +, CD13 +, CD19 +, CD20 +, CD34 +, CD37 +, CD38 +, cyCD79a +, CD80 -, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, sm/cykappa -, sm/cylambda -
Viruses:

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

TOM1

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.