TANOUE

Specification
Q & A
Customer Review

Cat.No.

CSC-C0445

Description

Established from the peripheral blood of an 11-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in 1990

Species

Homo sapiens (Human)

Source

Blood; Peripheral Blood

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, small, round cells growing in suspension

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 47/48<2n>X, -Y/XYqh+, +7, +14, dup(1)(q21.1/21.2q23.1/23.2), t(2;4)(q2?2;q2?5), del(6)(q27), t(8;14)(q24;q32), del(13)(q34), add(22)(q13) - extensive subclonal variation - carries t(8;14), +7, and dup(1q)

Disease

Childhood B Acute Lymphoblastic Leukemia

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 (+), CD38 +, cyCD79a +, CD138 +, HLA-DR (+)
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HB

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

Tanoue

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Ask a Question

Write your own review

You May Also Need

CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.