NAMALWA.KN2

Cat.No.: CSC-C0229

Species: Homo sapiens (Human)

Morphology: lymphoblastoid, single cells with irregular forms or small clusters, in suspension

Culture Properties: Suspension

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Cat.No.

CSC-C0229

Description

The NAMALWA.KN2 cell line is a subclone of the cell line NAMALWA. Established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA was described to be characterized by differential expression of immunoglobulin and MYC genes and of some cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H confirmed as human with IEF of AST, MDH, NP.

Species

Homo sapiens (Human)

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

Suspension

Morphology

lymphoblastoid, single cells with irregular forms or small clusters, in suspension

STR DNA Profile

Amelogenin: X
CSF1PO: 10,11
D13S317: 12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9,3
TPOX: 6,11
vWA: 14

Karyotype

Human hypodiploid karyotype with 7% tetraploidy - 45(41-45)<2n>X, -Y - NAMALWA marker chromosomes present

Disease

EBV-Related Burkitt Lymphoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +-
Viruses: ELISA: reverse trans

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

Namalwa.KN2; Namalwa KN2; KN2

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.