The NAMALWA.KN2 cell line is a subclone of the cell line NAMALWA. Established from the tumor mass of an African child with Burkitt lymphoma; this variant subline from the original NAMALWA was described to be characterized by differential expression of immunoglobulin and MYC genes and of some cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H confirmed as human with IEF of AST, MDH, NP.
Homo sapiens, human
STR DNA Profile
Human hypodiploid karyotype with 7% tetraploidy - 45(41-45)<2n>X, -Y - NAMALWA marker chromosomes present
lymphoblastoid, single cells with irregular forms or small clusters, in suspension
Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +-
Viruses: ELISA: reverse trans
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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