COLO-699

Cat.No.: CSC-C0321

Species: Homo sapiens (Human)

Source: Pleural Effusion Metastasis

Morphology: adherent, epitheloid cells growing as monolayers

Culture Properties: monolayer

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Cat.No.

CSC-C0321

Description

Established from the pleural fluid of a 57-year-old woman with adenocarcinoma of the lung in 1986

Species

Homo sapiens (Human)

Source

Pleural Effusion Metastasis

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

monolayer

Morphology

adherent, epitheloid cells growing as monolayers

Karyotype

Human hypertriploid karyotype with 2% polyploidy - 70(62-72)<3n>XX, -X, +1, +3, -4, +6, +7, +7, +8, -9, -10, +12, -14, -16, -17, -17, +18, +20, der(2)t(2;10)(q27;q12), der(7)t(7;?)(p21;?)x2, i(10q), der(11)t(8;11)(q12;q14), idic(16q), der(21)t(21;?)(p12;?

Disease

Lung Adenocarcinoma

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: cytokeratin -, cytokeratin-7 -, cytokeratin-8 -, cytokeratin-17 -, cytokeratin-18 -, desmin -, endothel -, EpCAM -, GFAP -, neurofilament -, vimentin +; cells are

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

COLO 699; Colo-699; COLO #699; Colo699; COLO699; Colorado 699; CHL1DM

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.