H-209

Specification
Recommended Products

Cat.No.

CSC-C0510

Description

Established in 1979 from the bone marrow aspirated from a 55-year-old white man with small cell lung carcinoma prior to treatment; corresponds to NCI-H209; described as expressing neuroendocrine biochemical markers, e.g. neuron-specific enolase, brain creatine kinase, L-DOPA decarboxylase and bombesin

Species

Human

Source

small cell lung carcinoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Morphology

spherical-to-ovoid cells growing singly in large clumps

Karyotype

Human hyperdiploid karyotype with 8% polyploidy - 47(44-48)<2n>XY, -4, -5, +6, +7, +8, -10, -14, -15, -17, +0-2mar, r/dm, add(1)(p32-34), add(3)(p11), add(4)(q24-25), add(5)(p1?), add(6)(q14)x1-2, add(8)(p1?), add(17)(p1?) - extensive subclonal variation

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimentin -
Viruses: PCR

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

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CM-1448Y

SuperCult® RPMI 1640 Medium

INQUIRY

CS-001Y

PremSera® FBS

INQUIRY

For research use only. Not for any other purpose.