Subclone of the cell line GH3; established from a pituitary tumor induced in a female Wistar/Furth rat in 1965; described to produce a high rate of prolactin and a low rate of growth hormone synthesis
mostly round cells growing singly and in clusters in suspension and partially adherent; quality and amount of serum may influence the adherence of the cells
80-90% Hams F10 + 10-20% h.i. FBS + 2 mM L-glutamine (originally grown with 15% horse serum + 2.5% h.i. FBS in Ham′s F10)
Mycoplasma: contamination was eliminated with enrofloxacin (Baytril), then negative in microbiological culture, RNA hybridization, PCR assays
Viruses: PCR: SMRV -
Storage and Shipping
Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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