COLO-320

Cat.No.: CSC-C0300

Species: Homo sapiens (Human)

Source: Intestine; Colon

Morphology: undifferentiated, round cells in suspension and loosely adherent in monolayers

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Cat.No.

CSC-C0300

Description

Established from the tumor mass of a 55-year-old Caucasian woman with a moderately undifferentiated adenocarcinoma of the sigmoid colon in 1977; cells were described to produce several hormones, e.g. serotonin, epinephrine; cells express MYC mRNA

Species

Homo sapiens (Human)

Source

Intestine; Colon

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Morphology

undifferentiated, round cells in suspension and loosely adherent in monolayers

Karyotype

Human hyperdiploid karyotype - 53(50-54)<2n>XX, -3, -3, +5, +7, +12, -13, -17, +18, +22, +6mar, t(1;5) (p11;q11), der(5)t(5;?)(p15;?), der(7)t(7;?)(q22;?), der(9)t(9;?)(p11;?), del(9)(p22), del(12)(p12), i(13q), der(14)t(14;?)(q24;?), der(18)t(18;?)(p11;?

Disease

Colon Adenocarcinoma

Quality Control

Mycoplasma: contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, desmin -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Synonyms

COLO 320; Colo-320; Colo 320; COLO #320; COLO320; Colo320; CoLo320; Co320; COLO 320F; Colorado 320

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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