Sprague-Dawley (SD) Rat Mesenchymal Stem Cells with GFP

Sprague-Dawley (SD) Rat Mesenchymal Stem Cells with GFP are primary bone marrow-derived mesenchymal stem cells (BMSCs) obtained from the femur/tibia of healthy SD rats and stably transduced with a lentiviral vector encoding Green Fluorescent Protein (GFP) for real-time fluorescence tracking. These cells show typical MSC features namely, spindle shaped adherent growth, fibroblast-like form, expression of mesenchymal surface markers (CD29, CD44, and CD90 >70%) and lack of hematopoietic surface markers (CD34, CD45, and CD11b <5%), and the ability to differentiate in vitro into osteoblasts, adipocytes, and chondrocytes in vitro. These are usually cultivated in a basal nutritional medium supplemented with serum and growth factors, under standard conditions (37 °C, 5% CO₂) at a recommended seeding density of 2-4 × 10⁴ cells/cm², and passaged before over-confluence to maintain multipotency (limited to ~5-10 passages).

The integrated GFP reporter enables immediate visualization of cell survival, migration and homing following in vivo transplantation without further labeling. Thus, these GFP-labeled SD rat MSCs are widely used in the preclinical field to study cell tracking, engraftment efficiency, immunomodulatory, and paracrine effects in various rat disease models, including orthotopic bone/cartilage repair, spinal cord or myocardial infarction models, and so on.