Human iPSC-derived renal proximal tubular cells

Specification
Background
Scientific Data
Q & A
Customer Review

Cat.No.

CSC-00833L

Description

Human iPSC-Derived Renal Proximal Tubular Cells are derived from integration-free iPSCs of a healthy donor and have been differentiated through the up-regulation of specific signalling pathways. To support the growth and maintenance of Human iPSC-Derived Renal Proximal Tubular Cells, we offer our Renal Epithelial Cell Culture Medium which is fully supplemented with hormones and recombinant growth factors that promote optimal growth. Our iPSC-Derived Renal Proximal Tubular Cells express critical functional markers, along with epithelial and renal cell markers. These include the important phosphate transporter, SLC34A1, the organic ion transporter crucial for baso-lateral uptake of organic anions, OAT1 and the apical glucose transporter SGLT2, key in glucose reabsorption from the glomerular filtrate. These cells provide a physiologically relevant model for investigating glucose uptake in diabetes, the transportation of drugs and endogenous metabolites, and detecting nephrotoxicity.

Species

Human

Recommended Medium

Human iPSC-derived Renal Epithelial Cell Culture Media

Application

For Research Use Only

Shipping

Dry Ice

Storage and Shipping

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

hiPSC-derived renal proximal tubular cells (hiPSC-derived PTCs/PTLs) are functional epithelial-like cells that are created by guided differentiation of human induced pluripotent stem cells (hiPSCs) into the renal proximal tubule lineage. The cells show significant morphological and physiological properties of natural proximal tubular epithelial cells (PTECs) and serve as a viable in vitro model for kidney research and translational toxicology.

hiPSC-derived proximal tubular cells have conventional epithelial shape and express typical proximal tubule markers, including megalin (LRP2), cubilin, AQP1 and brush border-type transport proteins. Functionally, they exhibit albumin endocytosis, the activity of transporters (OATs, OCTs) and polarized epithelial characteristics which are required to imitate renal reabsorption and xenobiotic processing in vivo.

More recently, these cells have been shown to respond in a physiologically realistic manner to known nephrotoxins such as cisplatin, cyclosporine A and aminoglycosides, supporting their application in nephrotoxicity screening and medication safety assessment. Furthermore, proximal tubular models created from hiPSCs are being included into kidney organoid systems and microfluidic organ-on-chip platforms, allowing for increased maturation, enhanced transporter expression and more faithful modelling of renal physiology.

In summary, human iPSC-derived renal proximal tubular cells provide a robust and scalable human-relevant model to study renal development, drug-induced kidney injury, transporter biology and disease mechanisms, bridging the gap between the conventional cell lines and primary human renal epithelial cells.

Characterization of hiPSC Differentiation into Proximal Tubule Epithelial Cells

Mboni-Johnston et al. investigated the sensitivity of human induced pluripotent stem cells (hiPSC) and their differentiated progeny to nephrotoxins. Dermal fibroblast-derived hiPSC lines (F4 and b4) were differentiated into proximal tubular epithelial-like cells (PTELC) following the protocol outlined in Figure 1A.

During differentiation, both cell lines transitioned morphologically from a cobblestone to a spindle-like shape by day 9, forming domes and tubule-like structures (Fig. 1B-D). Proliferation rates, assessed by EdU incorporation, dropped sharply in F4 cells from nearly 100% to approximately 10% by day 9 (Fig. 1E), whereas b4 cells exhibited a slower decline, retaining about 30% proliferation at day 9 (Fig. 1F).

Gene expression analysis revealed significant downregulation of stem cell markers (NANOG, Oct-3/4) in both lines after nine days, with Sox-2significantly reduced only in F4 (Fig. 2A). Conversely, epithelial markers like E-cadherin increased. Among four proximal tubule markers, all were significantly elevated in differentiated b4 cells, while only three (Aqp-1, Cad-16, Glut-5) increased significantly in F4 cells, with CD13mRNA unchanged. Immunocytochemistry confirmed these changes at the protein level (Fig. 2B), revealing discrepancies between mRNA and protein expression for CD13 and Zo-1. Flow cytometry demonstrated that nearly all differentiated F4 cells expressed the PTEC marker Aquaporin-1 by day 9, a threefold increase over undifferentiated controls (Fig. 2C).

Differentiation protocol and physiological changes provoked by it. — Fig. 1. Differentiation protocol and physiological changes provoked by it (Mboni-Johnston I M, Kouidrat N M Z, *et al.*, 2023).

Fig. 2. Changes in expression of differentiation markers in F4- and b4-hiPSC differentiated into proximal tubular epithelial cell-like cells (PTELC) (Mboni-Johnston I M, Kouidrat N M Z, *et al.*, 2023).

Ask a Question

Write your own review

For research use only. Not for any other purpose.