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Cell Biology
- How to Handle Mycoplasma in Cell Culture?
- Enrichment, Isolation and Characterization of Circulating Tumor Cells (CTCs)
- Strategies for Enrichment of Circulating Tumor Cells (CTCs)
- How to Assess the Migratory and Invasive Capacity of Cells?
- Multi-Differentiation of Peripheral Blood Mononuclear Cells
- Tips For Cell Cryopreservation
- What Cell Lines Are Commonly Used in Biopharmaceutical Production?
- Troubleshooting Cell Culture Contamination: A Comprehensive Guide
- How to Isolate and Analyze Tumor-Infiltrating Leukocytes?
- Contamination of Cell Cultures & Treatment
- Generation and Applications of Neural Stem Cells
- Stem Cell Markers
- T Cell Activation and Expansion
- Comparison of the MSCs from Different Sources
- Organoid Differentiation from Induced Pluripotent Stem Cells
- Quantification of Cytokines
- Mesenchymal Stem Cells: A Comprehensive Exploration
- What are the Differences Between M1 and M2 Macrophages?
- How to Decide Between 2D and 3D Cell Cultures?
- Neural Differentiation from Induced Pluripotent Stem Cells
- Circulating Tumor Cells as Cancer Biomarkers in the Clinic
- CFU Assay for Hematopoietic Cell
- Monocytes vs. Macrophages
- How to Detect and Remove Endotoxins in Biologics?
- Comparison of Different Methods to Measure Cell Viability
- CHO Cell Line Development
- How to Isolate PBMCs from Whole Blood?
- What are PBMCs?
- How to Start Your Culture: Thawing Frozen Cells
- Biomarkers and Signaling Pathways in Tumor Stem Cells
- Techniques for Cell Separation
- Guidelines for Cell Banking to Ensure the Safety of Biologics
- Tumor Stem Cells: Identification, Isolation and Therapeutic Interventions
- Isolation, Expansion, and Analysis of Natural Killer Cells
- Unveiling the Molecular Secrets of Adipogenesis in MSCs
- T Cell, NK Cell Differentiation from Induced Pluripotent Stem Cells
- Major Problems Caused by the Use of Uncharacterized Cell Lines
- Critical Quality Attributes and Assays for Induced Pluripotent Stem Cells
- Direct vs. Indirect Cell-Based ELISA
- Cell Culture Medium
- What Is Cell Proliferation and How to Analyze It?
- IL-12 Family Cytokines and Their Immune Functions
- What are Mesothelial Cells?
- How to Scale Up Single-Cell Clones?
- STR Profiling—The ID Card of Cell Line
- Comparison of Several Techniques for the Detection of Apoptotic Cells
- What Are Myeloid Cell Markers?
- Cryopreservation of Cells Step by Step
- Cell Cryopreservation Techniques and Practices
- How to Eliminate Mycoplasma From Cell Cultures?
- Human Primary Cells: Definition, Assay, Applications
- Understanding Immunogenicity Assays: A Comprehensive Guide
- Role of Cell-Based Assays in Drug Discovery and Development
- Mastering Cell Culture and Cryopreservation: Key Strategies for Optimal Cell Viability and Stability
- Adherent and Suspension Cell Culture
- Spatial Metabolomics Sloution
- Organoid Drug Screening
- Histological Staining Techniques: From Traditional Chemical Staining to Immunohistochemistry
- Key Techniques in Primary, Immortalized and Stable Cell Line Development
- Overview of Cell Apoptosis Assays
- From Primary to Immortalized: Navigating Key Cell Lines in Biomedical Research
- Modern Histological Techniques
- From Specimen to Slide: Core Methods in Histological Practice
- Exploring Cell Dynamics: Migration, Invasion, Adhesion, Angiogenesis, and EMT Assays
- Cell Viability, Proliferation and Cytotoxicity Assays
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Histology
- Troubleshooting in Fluorescent Staining
- Tips for Choosing the Right Protease Inhibitor
- Multiple Animal Tissue Arrays
- Instructions for Tumour Tissue Collection, Storage and Dissociation
- Fluorescent Nuclear Staining Dyes
- Guides for Live Cell Imaging Dyes
- Overview of the FFPE Cell Pellet Product Lines
- Mitochondrial Staining
- Stains Used in Histology
- Immunohistochemistry Controls
- Comparison of Membrane Stains vs. Cell Surface Stains
- Overview of Common Tracking Labels for MSCs
- How to Apply NGS Technologies to FFPE Tissues?
- Microscope Platforms
- Cell Lysates: Composition, Properties, and Preparation
- Immunohistochemistry Troubleshooting
- Cell and Tissue Fixation
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Exosome
- Exosomes as Emerging Biomarker Tools for Diseases
- How to Apply Exosomes in Clinical?
- How to Efficiently Utilize MSC Exosomes for Disease Treatment?
- Summary of Approaches for Loading Cargo into Exosomes
- What's the Potential of PELN in Disease Treatment?
- Emerging Technologies and Methodologies for Exosome Research
- How to Perform Targeted Modification of Exosomes?
- How to Enhancement Exosome Production?
- How to Label Exosomes?
- How to characterize exosomes?
- Classification, Isolation Techniques and Characterization of Exosomes
- Exosome Quality Control: How to Do It?
- Common Techniques for Exosome Nucleic Acid Extraction
- Exosome Transfection for Altering Biomolecular Delivery
- Collection of Exosome Samples and Precautions
- How do PELN Deliver Drugs?
- Current Research Status of Milk Exosomes
- Exosome Antibodies
- How Important are Lipids in Exosome Composition and Biogenesis?
- Unraveling Biogenesis and Composition of Exosomes
- Applications of MSC-EVs in Immune Regulation and Regeneration
- Production of Exosomes: Human Cell Lines and Cultivation Modes
- The Role of Exosomes in Cancer
- What are the Functions of Exosomal Proteins?
- Exosome Size Measurement
- Techniques for Exosome Quantification
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ISH/FISH
- ISH probe labeling method
- What Types of Multicolor FISH Probe Sets Are Available?
- What Is the Use of FISH in Solid Tumors?
- Mapping of Transgenes by FISH
- Comprehensive Comparison of IHC, CISH, and FISH Techniques
- Telomere Length Measurement Methods
- Reagents Used in FISH Experiments
- FISH Tips and Troubleshooting
- Differences Between DNA and RNA Probes
- Overview of Oligo-FISH Technology
- Comparative Genomic Hybridization and Its Applications
- RNAscope ISH Technology
- CARD-FISH: Illuminating Microbial Diversity
- What are the Differences between FISH, aCGH, and NGS?
- Multiple Options for Proving Monoclonality
- FISH Techniques for Biofilm Detection
- Whole Chromosome Painting Probes for FISH
- Overview of Common FISH Techniques
- What are Single, Dual, and Multiplex ISH?
- Multiple Approaches to Karyotyping
- In Situ Hybridization Probes
- Different Types of FISH Probes for Oncology Research
- How to Use FISH in Hematologic Neoplasms?
- Small RNA Detection by ISH Methods
- Guidelines for the Design of FISH Probes
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Toxicokinetics & Pharmacokinetics
- How to Improve Drug Plasma Stability?
- What Are Metabolism-Mediated Drug-Drug Interactions?
- How to Conduct a Bioavailability Assessment?
- Organ-on-a-Chip Systems for Drug Screening
- Experimental Methods for Identifying Drug-Drug Interactions
- Toxicokinetics vs. Pharmacokinetics
- How Is the Cytotoxicity of Drugs Determined?
- How to Improve the Pharmacokinetic Properties of Peptides?
- Pharmacokinetics Considerations for Antibody Drug Conjugates
- Key Considerations in Toxicokinetic
- Organoids in Drug Discovery: Revolutionizing Therapeutic Research
- Traditional vs. Novel Drug Delivery Methods
- Unraveling the Role of hERG Channels in Drug Safety
- Comparison of MDCK-MDR1 and Caco-2 Cell-Based Permeability Assays
- What factors influence drug distribution?
- How to Design and Synthesize Antibody Drug Conjugates?
- Methods of Parallel Artificial Membrane Permeability Assays
- Pharmacokinetics of Therapeutic Peptides
- What Is the Role of the Blood-Brain Barrier in Drug Delivery?
- Parameters of Pharmacokinetics: Absorption, Distribution, Metabolism, Excretion
- What are the Pharmacokinetic Properties of the Antisense Oligonucleotides?
- Key Factors Influencing Brain Distribution of Drugs
- How to Improve Drug Distribution in the Brain
- Physical and Chemical Properties of Drugs and Calculations
- Effects of Cytochrome P450 Metabolism on Drug Interactions
- What Are Compartment Models in Pharmacokinetics?
- The Rise of In Vitro Testing in Drug Development
- Predictive Modeling of Metabolic Drug Toxicity
- Overview of In Vitro Permeability Assays
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Disease Models
- What Human Disease Models Are Available for Drug Development?
- Overview of Cardiovascular Disease Models in Drug Discovery
- Summary of Advantages and Limitations of Different Oncology Animal Models
- Preclinical Models of Acute Liver Failure
- Animal Models of Neurodegenerative Diseases
- Why Use PDX Models for Cancer Research?
- Disease Models of Diabetes Mellitus
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Cell Quantification Protocol
GUIDELINE
Cell quantification refers to the process of determining the number or concentration of cells in a given sample. The protocol describes a method of cell quantification.
METHODS
- Bring adherent and semi-adherent cells into suspension using trypsin/EDTA and resuspended in a volume of fresh medium at least equivalent to the volume of trypsin. For cells that grow in clumps centrifuge and resuspend in a small volume and gently pipette to break up clumps.
- Before the cells settle, place a suitable volume of a cell suspension (20-200 μL) in a centrifuge tube. Add an equal volume of 0.4% Trypan Blue and gently mix. Incubate the mixture at room temperature (15-25°C) for 5 minutes.
- Prepare a hemocytometer by cleaning the chamber surface with 70% ethanol.
- Moisten the coverslip with water or exhaled breath. Fill both sides of the chamber with cell suspension (approximately 5-10 μL) and view under an inverted phase contrast microscope using x20 magnification.
- Count the number of viable (seen as bright cells) and non-viable cells (stained blue). Ideally >100 cells should be counted in order to increase the accuracy of the cell count. Note the number of squares counted to obtain your count of >100.
- Calculate the concentration of viable and non-viable cells and the percentage of viable cells.
Creative Bioarray Relevant Recommendations
- Creative Bioarray provides Trypan blue staining, commonly used for the determination of cell viability. And we provide many fluorescent dyes that are highly specific to a variety of organelles and can be used to monitor cell health and cell death. Our cell counting kit also performs well in practice. We are always committed to providing customers with high-quality products and services.
NOTES
- Trypan Blue is toxic and is a potential carcinogen. Protective clothing, gloves, and face/eye protection should be worn. Do not breathe the vapor.
- The central area of the counting chamber is 1 mm2. This area is subdivided into 25 smaller squares (1/25 mm2). Each of these is surrounded by triple lines and is then further divided into 16 (1/400 mm2). The depth of the chamber is 0.1 mm.
- Be careful not to move the coverslip. Allow capillary action to draw the sample in.
For research use only. Not for any other purpose.