this is a spontaneously arising retinal pigment epithelia (RPE) cell line derived from the normal eyes of 19-year-old male who died from head trauma in a motor vehicle accident; the cells form stable monolayers, which exhibit morphological and functional polarity; the line expresses the RPE-specific markers CRALBP and RPE-65; the cells exhibit morphological polarization when plated on laminin-coated Transwell-COL filters in medium with a low serum concentration; they form tight-junctions with transepithelial resistance of monolayers reaching a maximum of 50 to 100 ohms/cm2 after 4 weeks of culture; the cells are diploid and can be carried for over 30 passages.
Storage and Shipping
liquid nitrogen vapor phase
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.