Species: mouse - Mus musculus, Swiss
Note: Cells undergo a pre-adipose to adipose like conversion as they progress from a rapidly dividing to a confluent and contact inhibited state. A high serum content in the medium enhances fat accumulation; tested and found negative for ectromelia virus (mousepox); the cells should be grown in plastic flasks, they do not grow well on some types of glass surfaces; a saturation density of approximately 50000 cells per sq cm can be reached.
Tests for mycoplasma, bacteria and fungi were negative
Storage and Shipping
Frozen with 52.5% RPMI-1640, 40% FBS, 7.5% DMSO at about 4-5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
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