Porcine Esophageal Epithelial Cells

Specification
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Cat.No.

CSC-C9244J

Description

Porcine Esophageal Epithelial Cells from Creative Bioarray are isolated from esophageal tissue of porcine. Porcine Esophageal Epithelial Cells are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6 cells per ml and is delivered frozen. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.

Species

Porcine

Source

Esophagus

Recommended Medium

Complete Epithelial Cell Medium

Cell Type

Epithelial

Disease

Normal

Storage and Shipping

We ship frozen cells on dry ice. Upon receiving, directly and immediately transfer the cells from dry ice to liquid nitrogen and keep the cells in liquid nitrogen until they are needed for experiments. Never can primary cells be kept at -20 °C.

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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For research use only. Not for any other purpose.

Q & A
Customer Review

What should I do if I find flocculent precipitates after thawing the serum?

There are many reasons for the appearance of precipitates in serum, but the most common cause is due to the denaturation of lipoproteins in serum, while blood fibrin (one of the proteins that form clots) is also present in serum after it has been thawed and is also a cause of precipitates. However, these flocculent precipitates do not affect the quality of the serum itself. To remove these flocculent deposits, the serum can be divided into sterile centrifuge tubes and centrifuged slightly at 400 g. The supernatant can then be added to the culture medium and filtered together. It is best not to use filtration to remove these flocculent sediments as it may clog the filter membrane.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

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The development of this product shows a global leadership in innovation and the products we use are the latest.

02 Apr 2023

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