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Human Oral Keratinocytes (HOK)

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Cat.No.
CSC-7745W
Description
Human Oral Keratinocytes (HOK) from Creative Bioarray are isolated from human oral mucosa. HOK are cryopreserved on passage one culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HOK are characterized by immunofluorescent method with antibodies to cytokeratine-8, -18 and -19. HOK are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HOK are guaranteed to further expand for 15 population doublings in the condition provided by Creative Bioarray.
Species
Human
Source
Oral Cavity
Cell Type
Keratinocyte
Disease
Normal
Storage and Shipping
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
Q1:
I would like to know if this cell line is immortalized and if I can expand it for experiments involving analysis of DNA damage (eg. comet and TUNEL assays) and cellular transformation (eg. soft agar colony formation assay). Furthermore, I would like to know if CSC-7745W Human Oral Keratinocytes (HOK) were isolated from gingiva. If not, where do they come from?
A:

Human Oral Keratinocytes (HOK) are primary cells, but we do also have Immortalized Human Oral Keratinocytes in stock. They are both isolated from human gingiva tissues.

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