Hepatic stellate cells (HSteC) are intralobular connective tissue cells presenting myofibroblast-like or lipocyte phenotypes. They participate in the homeostasis of liver extracellular matrix, repair, regeneration, fibrosis and control retinol metabolism, storage and release. Following liver injury, HSteC transform into myofibroblast-like cells and are the major source of type I collagen in the fibrotic liver. Beyond these feature, HSteC have been implicated as regulators of hepatic microcirculation via cell contraction, and in disease states, in the pathogenesis of intrahepatic portal hypertension. Proliferation and migration of HSteC and expression of chemokines are involved in the pathogenesis of liver inflammation and fibrogenesis. HSteC possess voltage-activated calcium current, express the low affinity nerve growth factor receptor p75, and undergo apoptosis in response to nerve growth factor stimulation. Therefore, the new insight into the molecular regulation of HSteC activation will lead to therapeutic approaches in treatment of hepatic fibrosis in the future, and could lead to reduced morbidity and mortality in patients with chronic liver injury.
HHSteC are isolated from human liver. HHSteC are cryopreserved after purification and delivered frozen. HHSteC are characterized by immunofluorescent method with antibodies to desmin and α-actin. HHSteC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HHSteC are guaranteed to further expand for 15 population doublings in the conditions provided by Creative Bioarray.
It is recommended to use Stellate Cell Medium for the culturing of HHSteC in vitro.
Storage and Shipping
ship in dry ice; store in liquid nitrogen
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.