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Human Cardiomyocytes

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Cat.No.
CSC-C2847
Description
Cardiomyocytes are derived from the whole heart (of a single donor) that has been dissociated into single cells and cultured using differential adhesion. These cells are characterized by immunostaining of cardiac sarcomeric actin, myosin heavy chain and other cardiac markers.

Cardiomyocytes may be used in various in vitro or in vivo studies; transplantation into normal or diseased systems; cardiac toxicology studies; or cardiovascular developmental studies.
Storage and Shipping
ship in dry ice; store in liquid nitrogen
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Q1:
Do Human Cardiomyocytes (CSC-C2847) proliferate and what is the number of passages guaranteed?
A:

Actually, we have two types of Human Cardiomyocytes: tissue-derived and iPSC-derived. The tissue-derived cardiomyocytes are isolated from normal human ventricle tissue of the adult heart. They are guaranteed for 15 population doublings with a doubling time of 34.5h. The cells are cryopreserved at P2, so they'll be P3 when you start culture them. These cardiomyocytes don't beat in the culture. The iPSC-derived cardiomyocytes can be maintained in culture for an extended duration. We have cultured the cells for 1 year and the cells still maintain the beating phenotype and gene expression patterns.

Q2:
We are looking for cardiomyocytes (CSC-C2847) that maintain beta 1 adrenergic receptor expression during culture. Could you please send me some more information about your cells?
A:

We can provide two kinds of human cardiomyocytes, tissue-derived and iPSC-derived. However, we haven't tested them for beta 1 adrenergic receptor expression.

Q3:
Where is the tissue-derived cardiomyocytes (CSC-C2847) coming from atrial or ventricle?
A:

The tissue-derived cardiomyocytes are isolated from normal human ventricle tissue of the adult heart.

Q4:
Each time I order primary cardiomyocytes, would they be from a different individual?
A:

Since the cells are isolated from donated tissues and the amount of cells from a single donor is limited, we run out of the cells from the same donor every now and then. Therefore, we cannot guarantee that the cells you order each time will from the same donor. We might be able to reserve 1 or 2 vials for you when you place your first order, but the reservation can stay valid for a month or two.If it's necessary for you to use cardiomyocytes continuously from the same origin, I would like to recommend to you our cardiomyocytes derived from iPS cells. These cells are quite stable and they are able to maintain their beating morphology if cultured with our optimized media.

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CONTACT US USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
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Europe
Tel: 44-207-097-1828
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