The development of the cerebellum involves a set of coordinated cell movements and two separate proliferation zones: the ventricular zone and the external granule cell layer (EGL), a rhombic-lip-derived progenitor pool. The EGL appears to be segregated during early cerebellum formation and produces only granule cells. Cerebellar granule cells (CGC) are the most abundant neurons of the brain. Their axons run as parallel fibres along the coronal axis, and the one-dimensional spread of excitation that is expected to result from this arrangement is a key assumption in theories of cerebellar function. CGC receive inhibitory synaptic input from Golgi cells, which are mediated by gamma-aminobutyric acid (GABA). During both in vivo and in vitrodevelopment, CGC depend on the activity of the NMDA glutamate receptor subtype for survival and full differentiation. Cultured CGC are widely used as a model system for studying neuronal apoptosis.
RCGC are isolated from neonate day 8 rat cerebellum. RCGC are cryopreserved at primary culture and delivered frozen. Each vial contains >1 x 10^6 cells in 1 ml volume. RCGC are characterized by immunofluorescent method with antibodies to neurafilament, MAP2, and beta-tubulin 3. RCGC are negative for mycoplasma, bacteria, yeast and fungi. RCGC are guaranteed to further culture in the conditions provided by Creative Bioarray.
It is recommended to use Neuronal Medium for the culture of RCGC in vitro.
Storage and Shipping
ship in dry ice; store in liquid nitrogen
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