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Human Colonic Epithelial Cells

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Cat.No.
CSC-C1488
Description
The colorectum is a major organ for both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host's internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colon epithelial cells can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo. They also respond to a broad array of cytokines with altered gene expression and growth characteristics. In addition, normal colonic epithelial cells were found to express the integrin α3, α5, α6, β1 and β4 chains.

HCoEpiC are isolated from human colonic tissue. HCoEpiC are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume. HCoEpiC are characterized by immunofluorescent method with antibodies to CK18 and CK19. HCoEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HCoEpiC are guaranteed to further culture for 10 population doublings in the conditions provided by Creative Bioarray.
Species
Human
Organism
Human
Source
Colon
Recommended Medium
It is recommended to use Colonic Epithelial Cell Medium for the culturing of HCoEpiC in vitro.
Storage and Shipping
ship in dry ice; store in liquid nitrogen
Cell Type
Epithelial Cells
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
Q1:
what is the expected number per vial?
A:

Each vial contains at least 0.5 x 10^6 cells cells.

Q2:
May you let us know whether the following cells were transfected after isolation or somehow transformed or immortalized?
A:

Human Colonic Epithelial Cells are primary cells, which are isolated from tissues. And we can also provide the Immortalized Human Colonic Epithelial Cells for you.

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