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Human Bronchial Smooth Muscle Cells (HBSM)
Cat.No.: CSC-7821W
Species: Human
Source: Bronchus
Cell Type: Smooth Muscle Cell
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Airway smooth muscle is fundamental for maintaining airway tone. The specialized ultrastructural features and regulatory mechanisms of airway smooth muscle are vital for normal airway function. Patients with respiratory disorders, such as severe asthma, exhibit the distinguishing feature of increased bronchial smooth muscle mass. The increase in smooth muscle mass is related either to abnormal bronchial smooth muscle cell (BSMC) proliferation or the accumulation of contractile protein. Smooth muscle cell proliferation is an important component of airway remodeling in asthma. It is consequently a target for the development of novel anti-asthma agents. Airway smooth muscle cells express CT-1 and release GM-CSF, RANTES and IL-8 in vitro. In order to develop therapies for asthma, researchers have utilized Human Bronchial Smooth Muscle Cells to elucidate the cellular and molecular mechanisms underlying BSMC proliferation.
Fig. 1. Airway cellular pathophysiology (Abohalaka, Reshed. 2023).
Creative Bioarray provides high quality Human Bronchial Smooth Muscle Cells (HBSMC), which are isolated from human bronchi and bronchioles and cryopreserved at P2, with >0.5 million cells in each vial. HBSMC express α-smooth muscle actin and desmin and are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fung. HBSMC can further expand in Smooth Muscle Cell Growth Medium (Cat# CM-1194W) under the condition suggested by Creative Bioarray.
Diseased Human Bronchial Smooth Muscle Cells are also available from donors diagnosed with asthma, COPD and cystic fibrosis. Detailed donor information for these cells can be obtained by contacting our scientific support team.
Zedoarondiol Inhibits Human Bronchial Smooth Muscle Cell Proliferation Through the CAV-1/PDGF Signaling Pathway
Airway remodeling in lung diseases can be treated by inhibiting excessive smooth muscle cell proliferation. Zedoarondiol (Zed) is a natural compound isolated from the Chinese herb Curcuma longa. The caveolin-1 (CAV-1) is widely expressed in lung cells and plays a key role in platelet-derived growth factor (PDGF) signaling and cell proliferation. This study aims to investigate the effect of Zed on human bronchial smooth muscle cell (HBSMC) proliferation and explore its potential molecular mechanisms. We assessed the effect of Zed on the proliferation of PDGF-stimulated HBSMCs and performed proteomic analysis to identify potential molecular targets and pathways.
Fig. 1. Zedoarondiol induces cell apoptosis in HBSMCs and blocks proliferation of HBSMCs induced by PDGF-BB in G1 phase (Lyu, Yinglan, et al. 2024).
Fig. 2. Quantitative proteomic analysis of the action of Zed on PDGF-BB-induced proliferation in HBSMCs (Lyu, Yinglan, et al. 2024).
Nanoplastics Penetrate Human Bronchial Smooth Muscle Cells and Affect Mitochondrial Metabolism
Micro- and nanoplastic particles, including common forms like polyethylene and polystyrene, have been identified as relevant pollutants, potentially causing health problems in living organisms. The mechanisms at the cellular level largely remain to be elucidated. This study aims to visualize nanoplastics in bronchial smooth muscle (BSMC) and small airway epithelial cells (SAEC), and to assess the impact on mitochondrial metabolism.
Healthy and asthmatic human BSMC and SAEC in vitro cultures were stimulated with polystyrene nanoplastics (PS-NPs) of 25 or 50 nm size, for 1 or 24 h. Live cell, label-free imaging by holotomography microscopy and mitochondrial respiration and glycolysis assessment were performed. The 25 and 50 nm NPs were shown to penetrate SAEC, along with healthy and diseased BSMC, and they impaired bioenergetics and induce mitochondrial dysfunction compared to cells not treated with NPs, including changes in oxygen consumption rate and extracellular acidification rate.
Fig. 3. The impact of polystyrene nanoplastics (PS-NPs) on the mitochondrial metabolism in human healthy bronchial smooth muscle cells (BSMC) and asthmatic (diseased) bronchial smooth muscle cells (DBSMC) (Winiarska, Ewa, et al. 2024).
The assessment included measurements of oxygen consumption rate (OCR), extracellular acidification rate (ECAR), and total proton efflux rate (PER) under basal conditions, as well as in response to the following mitochondrial inhibitors: oligomycin (Oligo), cyanide-4-(trifluoromethoxy)phenylhydrazone (FCCP), and a combination of rotenone and antimycin A (Rot/AA).
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Description: HTSMC from Creative Bioarray Research are isolated from human trachea. HTSMC are cryopreserved at secondary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HTSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HTSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTSMC are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: HPAAF from Creative Bioarray are isolated from human pulmonary artery. HPAAF are cryopreserved at passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HPAAF are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HPAAF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPAAF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Description: Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) provided by Creative Bioarray are isolated from the normal human pulmonary artery tissue. The cells are cryopreserved at passage 2 and delivered frozen. Each vial contains more than 0.5*10^6 viable cells after thawing. The cells are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HPASMCs are guaranteed for at least 12 population doublings under the conditions provided by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Description: Creative Bioarray's normal Human Lung Smooth Muscle Cells, when grown in SMC Medium, provide an ideal culture model for the study of angiogenesis, atherosclerosis, diabetes or vascular/pulmonary biology.
Cell Features:
HSMC are cryopreserved as secondary cells, e.g. cells are isolated from the stated tissue and expanded in culture vessels twice before cryopreservation.
HAoSMC are isolated from human aorta (ascending and/or descending).
HPASMC are isolated from human pulmonary artery.
HCASMC are isolated from human coronary arteries.
HLMSC are isolated from the lobes of the lungs.
HBTSMC are isolated from the trachea and primary bronchi.
HSMC can be grown without phenol red or antimicrobials when cultured in VascuLife SMC Medium.
HSMC are extensively tested to meet quality standards and exhibit optimal performance.
Creative Bioarray guarantees performance and quality.
Description: Creative Bioarray's normal Human Lung Fibroblasts provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.
Cell Features:
HDFn are cryopreserved as primary cells. Cells are isolated from neonatal human foreskin and expanded in culture vessels once before cryopreservation.
HDFa are cryopreserved as primary cells. Cells are isolated from adult human skin and expanded in culture vessels once before cryopreservation.
Human Lung Fibroblasts are cryopreserved as secondary cells cells are isolated from human lungs and expanded in culture vessels twice before cryopreservation.
Fibroblasts can be grown in serum-free, animal protein-free, phenol red-free, and antimicrobial free conditions when cultured in FibroLife Serum-Free Medium.
Fibroblasts are extensively tested to meet quality standards and exhibit optimal performance.
Creative Bioarray's Fibroblasts are suitable for use as feeder layers as xeno-free or non-xeno free cultures for human embryonic stem cells and other cell culture applications requiring feeder layers.
Description: Creative Bioarray's normal Human Airway Epithelial Cells, when grown in Creative Bioarray's LIBro Medium, provide an ideal serum-free culture model for the accurate studies of toxicity, cystic fibrosis, asthma, pathogenesis, pharmacology or airway wound healing. Creative Bioarray's HSAEC are cultured without retinoic acid and cryopreserved as primary cells to ensure the highest viability and plating efficiency. Our HSAEC are quality tested in LIBro SAE Medium to ensure optimal growth over a period of at least 15 population doublings at rates equal to or greater than other commercially available media.
Cell Features:
HBTEC are cryopreserved as primary cells. Cells are isolated from human bronchi/trachea and expanded once in culture vessels before being harvested for cryopreservation.
HSAEC are cryopreserved after primary cells are isolated from human lung tissue and expanded once in culture vessels before being harvested for cryopreservation.
Creative Bioarray's Human Airway Epithelial Cells are not exposed to retinoic acid during isolation or expansion.
Human Airway Epithelial Cells are extensively tested for quality and optimal performance.
Creative Bioarray guarantees performance and quality.
