The most abundant cell type in the bronchus is fibroblasts. They resemble a mesenchymal stem cell phenotype and their principle function is the production of type III collagen, elastin, and proteoglycans of the extracellular matrix. Bronchial fibroblasts play an important role in the repair and remodeling processes following injury. The controlled accumulation of fibroblasts to sites of inflammation is crucial for effective tissue repair after injury. Either inadequate or excessive accumulation of fibroblasts can result in abnormal tissue function. For example, the excess fibroblast proliferation and collagen secretion that occurs from bronchial subepithelial fibrosis can result in airway obstruction and bronchial hyper-responsiveness.
HBF are isolated from human bronchus tissue. HBF are cryopreserved at secondary culture (P1) and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBF are characterized by immunofluorescent method with antibody to fibronectin. HBF are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBF are guaranteed to further expand for 15 population doublings at the condition provided by Creative Bioarray.
Human Primary Bronchial Fibroblasts are tested for negative expression of von Willebrand Factor Expression/Factor VIII, cytokeratin 18, and alpha smooth muscle actin. Human Primary Bronchial Fibroblasts are negative for bacteria, yeast, fungi and mycoplasma. Cells can be expanded for 3-5 passages at a split ratio of 1:2 or 1:3 under the cell culture conditions specified by Creative Bioarray.Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
We ship frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Live cell shipment is also available on request.
Never can primary cells be kept at -20 °C.
Human Primary Bronchial Fibroblasts can be used for the assay of cell-cell interaction, adhesion, PCR, Western blot, immunoprecipitation, immunofluorescent flow cytometry, or generating cell derivatives for desired research applications.
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