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ActoFactor™ Recombinant Human Thrombopoietin

  • Specification
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Thrombopoietin (TPO) is a key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in vivo. TPO stimulates the proliferation and maturation of megakaryocytes, and has also been shown to promote the survival, self-renewal and expansion of hematopoietic stem cells and primitive multilineage progenitors. Recombinant human TPO contains 332 amino acid residues and has a predicted molecular mass of approximately 35 kDa. As a result of glycosylation, the recombinant protein migrates with an apparent molecular mass of 47 kDa in SDS-PAGE.
Product Overview
Human THPO expressed in Sf21 insect cells
A DNA sequence encoding the mature human TPO protein sequence (Ser 22 - Gly 353; Accession #P40225) was expressed in Sf21 insect cells.
CAT# CSC-CTK0089-5 (5 μg); CAT# CSC-CTK0089-25 (25 μg)
Expression System
Sf21 insect cells
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The biological activity of recombinant human TPO is measured in a cell proliferation assay using MO7e cells. The ED50 for this effect is typically 0.3 - 3 ng/mL.
Endotoxin Level
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Recombinant human TPO is lyophilized from a 0.2 μm filtered solution in 35% acetonitrile, 0.1% trifluoroacetic acid containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitute human TPO at 50 μg/mL with sterile phosphate buffered saline (PBS) containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized human TPO is stable for up to twelve months from date of receipt at -20°C to -70°C. Reconstituted human TPO can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer) for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.
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