Human Interleukin 5 (IL-5) is a species-crossreactive cytokine produced by mast cells, T cells and eosinophils. It induces the proliferation, differentiation and activation of eosinophils. It is also a growth and differentiation factor for mouse but not human B cells. IL-5 is an antiparallel disulphide-linked homodimer of molecular mass of approximately 45 kDa. Mature IL-5 monomers contain 115 amino acid residues and 2 glycosylation sites. IL-5 binds to a specific heterodimeric cell surface receptor formed by two subunits, the binding IL-5Ra subunit and the non-binding b subunit, also shared by the receptors for IL-3 and GM-CSF, thus termed b-common (bc). Due to heterogeneous glycosylation, recombinant IL-5 resolves into multiple bands ranging from 32-34 kDa in SDS-PAGE.
Human IL5 expressed in Sf21 insect cells
A DNA sequence encoding the human IL-5 protein sequence was expressed in Sf 21 insect cells.
CAT# CSC-CTK0055-5 (5 μg); CAT# CSC-CTK0055-25 (25 μg)
Sf21 insect cells
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity was determined in a cell proliferation assay using a factor-dependent human cell line, TF-1 and the ED50 for this effect was typically 0.1 - 0.2 ng/mL.
Endotoxin level less than 0.1 ng per 1 mg of the cytokine as determined by the LAL method.
Lyophilized from a 0.2 μm filtered solution in PBS containing 50 mg of bovine serum albumin per 1 mg of cytokine.
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
Storage & Stability
Lyophilized samples are stable for greater than six months at -20°C to -70°C. Reconstituted IL-5 can be stored under sterile conditions at 2°C - 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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