Interleukin 3 (IL-3) is a species-specific pleiotropic cytokine, which can stimulate the proliferation and differentiation of pluripotent hematopoietic cells as well as various lineage-committed progenitors. Recombinant human IL-3 has a predicted molecular mass of approximately 15 kDa and is composed of the 133 amino acid residue mature form of IL-3 and the 134 amino acid residue methionyl form of IL-3 at a ratio of 1:2.
Human IL3 expressed in E.coli
A DNA sequence encoding the mature human IL-3 protein1 was expressed in E. coli.
CAT# CSC-CTK0078-10 (10 μg); CAT# CSC-CTK0078-50 (50 μg)
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
The biological activity of recombinant human IL-3 is measured in a cell proliferation assay using a human, factor-dependent cell line, TF-1. The ED50 for this effect is typically 0.1 - 0.4 ng/mL.
Endotoxin level is <1.0 EU per 1 μg cytokine, as determined by the LAL method.
Recombinant human IL-3 is lyophilized from a 0.2 μm filtered solution in phosphate buffered saline (PBS) containing 50 μg bovine serum albumin per 1 μg cytokine.
Reconstitute human IL-3 at a concentration greater than 10 μg/mL with sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage & Stability
Lyophilized human IL-3 is stable for upto twelve months from date of receipt at -20°C to -70°C. Reconstituted human IL-3 can be stored under sterile conditions at 2°C - 8°C for one month, or at -20°C to -70°C (in a manual defrost freezer)for three months without detectable loss of activity. Avoid repeated freezing and thawing.
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.