Immortalized Human Podocytes

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Immortalized Human Podocytes

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Cat.No.
CSC-I9325L
Description
Immortalized Human Podocytes are podocytes isolated from urine samples from healthy donor, and had been transformation with the thermosensitive SV40 large T antigen (U19tsA58) together with human telomerase (hTERT). The cells retained their primary cell characteristics with podocyte markers WT1, synaptopodin, and nestin. The urinary podocytes can be cultured for long-term and is a valuable tool for studies relating to pathogenesis and mechanisms of podocyte injury.
Organism
Homo sapiens
Source
Urinary Tissue
Culture Properties
Adherent
Morphology
Cuboidal
Immortalization Method
Co-infected with retrovirus carrying the thermosensitive SV40 large T antigen (U19tsA58) and with retrovirus carrying human telomerase (hTERT)
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid nitrogen until cell culture needed for experiments.

Note: Never can cells be kept at -20 °C.
Shipping
Dry Ice.
Recommended Products
CIK-HT013 HT® Lenti-hTERT Immortalization Kit
CIK-HT003 HT® Lenti-SV40T Immortalization Kit
Applications
For Research Use Only
Quality Control
1) Cultures were selected with 0.2 mg/ml G418 for T antigen, and 0.05 mg/ml hygromycin for hTERT;
2) Western blot preformed to determine SV40 T antigen expression
BioSafety Level
II
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the Pub Med ID of your paper to get a coupon.

For research use only. Not for any other purpose.

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